Avidity生物素蛋白連接酶-相關(guān)技術(shù)文章

更新時(shí)間：2024-07-11

瀏覽次數(shù)：1031

Avidity生物素蛋白連接酶

Avidity LLC 成立于 1996 年，位于色彩繽紛的科羅拉多州。

生wu素化菌株；AviTag 載體；生wu素化重組蛋白我們AviTag™技術(shù)是利用大腸桿菌的生wu素連接酶(BIRA)，在一個(gè)du特的15個(gè)氨基酸肽標(biāo)記上，對(duì)單一生wu素進(jìn)行高度靶向的酶結(jié)合。AviTag™序列為GLNDIFEAKIEWHE。定向在鏈霉親和素涂層表面，這創(chuàng)造了一個(gè)理想的表現(xiàn)分子結(jié)合相互作用。提供全套的蛋白、肽生wu素化的產(chǎn)品，其主要產(chǎn)品包括：生wu素蛋白連接酶（BirA）；生wu素化菌株；AviTag 載體；生wu素化重組蛋白。

以下是Avidity公司的部分熱銷(xiāo)產(chǎn)品

產(chǎn)品名稱(chēng)	貨號(hào)	規(guī)格
BirA Biotin-Protein Ligase	BIRA500	40ug: 2 vials of 20ug each
Bulk BirA Biotin-Protein Ligase	Bulk BIRA	300ug: 10 vials of 30ug each
Biotin Solution	BIO200	1.5 mL of 500uM d-biotin
Biotin Solution	BIO500	10 mL of 5 mM d-biotin
Positive Control Protein Substrate Kit	BIS300	1 Kit
BIS-300 positive and negative control protein kit	BIS-300	1 kit
Biotin AviTag™ Vectors (N-terminal)	pAN4	10 mg of plasmid DNA
Chemically Competent E. coli B Strain AVB101	CVB101	10 vials, 100 mL per vial
Efficient Biotinylation E. coli K12 Strain AVB100	AVB100	1 vial, containing 0.5 mL of glycerol stock
Anti-C-terminus AviTag™-conjugated Agarose Resin	AbCA	2.0 ml
Efficient Biotinylation E. coli B Strain AVB101	AVB101	1 vial, containing 0.5 mL of glycerol stock
Electrocompetent E. coli B Strain AVB101	EVB101	10 vials, 50 mL per vial
Electrocompetent E. coli K12 Strain AVB100	EVB100	10 vials, 50 mL per vial
Positive Control Plasmid	BIP300	2ug
Anti-C-terminus AviTag™ Antibody	AbC	100ul, 1 mg/ml
Biotinylated Gaussia princeps Luciferase	Gluc-10	10 ug
Biotinylated Gaussia princeps Luciferase	Gluc-100	100 ug
Streptavidin-conjugated Gaussia princeps Luciferase	SA-Gluc	50 ug
Native Coelenterazine Free Base	CTZ-1	1 mg
Biotinylated Renilla reniformis Green Fluorescent Protein	rrGFP-100	100 ug
Biotinylated Fluorescent Proteins(Twinkle-Avitag)	FP-495	100 ug
Biotinylated Fluorescent Proteins(Holly-Avitag)	FP-515	100 ug
Biotinylated Fluorescent Proteins(Dasher-Avitag )	FP-525	100 ug
Fluorescent Protein for detection of biotinylated molecules(SA-Twinkle)	SA-FP-495	100 ug
Fluorescent Protein for detection of biotinylated molecules(SA-Holly)	SA-FP-515	100 ug
Fluorescent Protein for detection of biotinylated molecules(SA-Cratchit)	SA-FP-545	100 ug
Fluorescent Protein for detection of biotinylated molecules(SA-Yukon)	SA-FP-563	100 ug
Fluorescent Protein for detection of biotinylated molecules(SA-Rudolph)	SA-FP-570	100 ug
Biotin AviTag™ Vectors (N-terminal)	pAN4, 5, or 6	10ug of plasmid DNA
Biotin AviTag™ Vectors (N-terminal)	pANs	one kit containing 10ug of each plasmid
Biotin AviTag™ Vectors (C-terminal)	pAC4, 5, or 6	10ug of plasmid DNA
Biotin AviTag™ Vectors (C-terminal)	pACs	one kit containing 10ug of each plasmid
Forward-sequence primer for Biotin AviTag™ Vectors	FASP	200 pmol
Reverse-sequence primer for Biotin AviTag™ Vectors	RASP	200 pmol